sewage virus DNA/RNA extraction kit

Sewage virus DNA/RNA extraction kit

utilizes a unique lysis solution and efficient decontamination technology, working in conjunction with grinding beads and a homogenizer to effectively break down the advanced structure of samples, ensuring the even release of nucleic acids from complex samples. It can extract viral DNA/RNA from enriched sewage samples, sediments, activated sludge, soil, feces, and other complex samples. Additionally, the magnetic bead capture system has been optimized to ensure successful capture of nucleic acids with smaller molecular weights, making it especially suitable for extracting and detecting pathogen nucleic acid fragments in sewage samples with low content and poor integrity. Through systematic optimization, this kit achieves effective sample lysis without the need for regulated chemicals such as β-mercaptoethanol, phenol, chloroform, or isoamyl alcohol, greatly expanding its applicability.

Description

Sewage virus DNA/RNA extraction kit utilizes a unique lysis solution and efficient decontamination technology, effectively breaking down complex samples to ensure even release of viral nucleic acids. The kit is specifically designed for extracting viral DNA/RNA from enriched sewage samples, sediments, activated sludge, soil, and feces, especially suitable for low-concentration or degraded pathogen nucleic acid fragments.

Through an optimized magnetic bead capture system, it ensures successful capture of low-molecular-weight nucleic acids without the need for hazardous chemicals such as β-mercaptoethanol or phenol, making the extraction process more environmentally friendly. The integrated inhibitor precipitation technology removes PCR inhibitors, ensuring high nucleic acid purity, ideal for downstream applications like PCR and RT-qPCR, which are sensitive to inhibitors.

Features:

  • High Efficiency: Automates the extraction of viral nucleic acids with low concentration and poor integrity from sewage.
  • High Purity: The inhibitor precipitation technology ensures that the extracted nucleic acids are pure and suitable for PCR, RT-qPCR, and other downstream experiments.
  • Environmentally Friendly: No use of regulated chemicals like β-mercaptoethanol, phenol, or chloroform, enhancing safety and applicability.
  • Wide Application: Suitable for extracting viral DNA/RNA from complex samples such as enriched sewage, sediments, activated sludge, soil, and feces.

Applications:

  • Environmental Monitoring: Extracting viral DNA/RNA from sewage samples, ideal for water quality testing and virus contamination monitoring.
  • Disease Surveillance: Used in public health for pathogen monitoring, especially during sewage treatment.
  • Research Applications: Supports viral research and epidemiological investigations with efficient viral nucleic acid extraction.

Parameters:

  • Sample Types: Sewage, sediment, activated sludge, soil, feces, and other complex samples.
  • Nucleic Acid Types: Viral DNA/RNA.
  • Nucleic Acid Extraction System: Magnetic bead capture.
  • Nucleic Acid Purity: High purity, suitable for PCR and RT-qPCR experiments.
  • Chemicals: Free of β-mercaptoethanol, phenol, chloroform, and other hazardous chemicals.
  • Direct Use in Downstream Experiments: PCR, RT-qPCR.

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